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Addiction is a health concern costing the country in both dollars and human resources. The realization that addictive behavior is a consequence of signaling disorders in the brain, and thus reflects a disease process, provides a basis to expect that effective treatments are possible. The binding of drugs and transmitters to cell membrane receptors initiates signaling pathways responsible for important components of drug addiction and addictive behaviors, and our laboratory has been involved in the study of these pathways. Small molecules, targeted to only a distinct receptor involved in addiction, will enable components of the many pathways resulting in addictive behavior to be untangled and provide a pharmacological rationale for designing novel therapies.
Over 40 G protein coupled receptors have been associated with addictive behaviors. We have collected these receptors and many of them have generated permanent cell lines containing 1) the receptor and beta-arrestin fluorescent protein biosensors [1, 2]; or 2) the MarsCy1-tagged receptor [3]. These cell lines can be used to create high content and low-to-high throughput assays to identify novel receptor ligands, or for studying the basic biology of the individual receptors. Our goal is to make the plasmids, cell lines, and data generated from them readily available to the NIDA/NIH funded research community. Thus, we hope this site will provide enabling tools that facilitate the work of the research community in understanding and combating addiction.
Use the navigational links above to view our catalog of plasmids and cell lines, as well as information on how to request a product.
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